10X Blocking Buffer from Abcam

Product Specs
Item10X Blocking Buffer
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab126587
Quantity50 ml
ApplicationsELISA, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, In-Cell ELISA, Western blot
TargetBlocking buffer

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Characterization of wooden breast myopathy: a focus on syndecans and ECM remodelingFront PhysiolDecember 5, 2023Lucie Pejšková, Sissel Beate Rønning, Matthew Peter Kent, Nina Therese Solberg, Vibeke Høst, To Thu-Hien, Jens Petter Wold, Marianne Lunde, Ellen Mosleth, Addolorata Pisconti, et al.by permeabilization with 0.1% Triton X-100 for 10 min. Samples were blocked in 1x blocking buffer (#ab126587, Abcam, United Kingdom) in PBS–T for 30 min and then stained using NucBlue Live Cell Stain Ready Probe

(2) Gradient Guided-Mode Resonance Biosensor with Smartphone ReadoutBiosensors (Basel)November 29, 2023Ting-Zhou Lin, Cheng-Hao Chen, Yuan-Pei Lei, Cheng-Sheng Huangantibodies. The channels were then blocked for 1 h at room temperature in a solution comprising 1% casein (ab126587, Abcam, Cambridge, UK) in PBS to minimize nonspecific binding and thus enable subsequent antigen binding.

(3) Increased epithelial mTORC1 activity in chronic rhinosinusitis with nasal polypsbioRxivOctober 21, 2023George X. Huang, Nils R. Hallen, Minkyu Lee, Kelly Zheng, Xin Wang, Michael V. Mandanas, Sarah Djeddi, Daniela Fernandez, Jonathan Hacker, Tessa Ryan, et al.positively charged glass slides (Fisher 12-550-15). Cryosections were blocked with 1x blocking buffer (Abcam ab126587) in PBST with 0.2% Triton X-100 and 0.1% Tween-20 for 1 h at room temperature. Afterwards, slides were

(4) Hexavalent Chromium Inhibited Zebrafish Embryo Development by Altering Apoptosis- and Antioxidant-Related GenesCurr Issues Mol BiolAugust 18, 2023Khoa Dang Dang, Chi Nguyen Quynh Ho, Huy Duc Van, Son Thanh Dinh, Quynh Thi Truc Nguyen, Tram Thi Thuy Nguyen, Xuyen Thi Ngoc Kien, Tuyet Van Dao, Hung Viet Nong, Minh Thai Nguyen, et al.Abcam, Cambridge, MA, USA), and the membrane was blocked overnight at 4 °C with a blocking buffer (ab126587, Abcam, Cambridge, MA, USA). The membrane was incubated with primary antibodies in a blocking buffer

(5) Ginsenoside Re prevents 3-methyladenine-induced catagen phase acceleration by regulating Wnt/β-catenin signaling in human dermal papilla cellsJournal of ginseng researchMay 1, 2023Jeong G, Shin SH, Kim SN, Na Y, Park BC, Cho JH, Park WS, Kim HJ.permeabilized with 0.3% Triton X-100 for 10 min. The cells were blocked with a blocking buffer (Abcam, England, ab126587) for 1 h and incubated with anti-LC3B antibody (1:200, Novus Biotechnology, NB100-2220) overnight at

(6) SIRT1/APE1 promotes the viability of gastric cancer cells by inhibiting p53 to suppress ferroptosisOpen Med (Wars)February 14, 2023Huijin Zhao, Yuanyi Ding, Lan Zhangroom temperature for 10 min, rinsed with PBS, and incubated in an immunofluorescence blocking buffer (ab126587; Abcam) at room temperature for 30 min. Thereafter, cells were incubated with anti-GPX4 antibody (1

(7) Two weeks of high glucose intake is enough to induce intestinal mucosal damage and disturb the balance of the gut microbiota of ratsBiomedical ReportsNovember 30, 2022Cunyun Min, Tingting Fu, Wei Tan, Tingting Wang, Yu Du, Xuhui HuangSDS-PAGE gel and transferred onto a PVDF membrane for 1 h at 100V. Subsequently, 10X Blocking Buffer (cat. no. ab126587; Abcam) was used to block the membrane to prevent non-specific background binding of the primary and

(8) Bufalin Inhibits Tumorigenesis, Stemness, and Epithelial-Mesenchymal Transition in Colorectal Cancer through a C-Kit/Slug Signaling AxisInternational journal of molecular sciencesNovember 1, 2022Ding L, Yang Y, Lu Q, Qu D, Chandrakesan P, Feng H, Chen H, Chen X, Liao Z, Du J, et al.separated by SDS-PAGE and transferred to a PVDF membrane. After blocking with 1× blocking buffer (#ab126587, Abcam, Cambridge, UK) for 2 h at RT, the membrane was incubated with primary antibody overnight at

(9) Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa CellsCurrent Issues in Molecular BiologyDecember 10, 2021Truong Xuan Dai et al.PVDF membrane (ab133411, Abcam, Cambridge, MA, USA). The membrane was treated with blocking buffer (ab126587, Abcam, Cambridge, MA, USA). The membrane was incubated with primary antibodies overnight at 4 °C. Anti-Cdk4

(10) Inhibition of DCLK1 with DCLK1-IN-1 Suppresses Renal Cell Carcinoma Invasion and Stemness and Promotes Cytotoxic T-Cell-Mediated Anti-Tumor ImmunityCancersNovember 16, 2021Ding L, Yang Y, Ge Y, Lu Q, Yan Z, Chen X, Du J, Hafizi S, Xu X, Yao J, et al.SDS-PAGE and transferred onto a PVDF membrane. The membrane was blocked with 1× blocking buffer (Abcam, ab126587; Cambridge, UK) for 1 h and then incubated with primary antibody at 4 °C overnight. Antibodies used

(11) Characteristics of Ventricular Electrophysiological Substrates in Metabolic Mice Treated with EmpagliflozinInternational Journal of Molecular SciencesJune 5, 2021Shih-Jie Jhuo, I-Hsin Liu, Wei-Chung Tasi, Te-Wu Chou, Yi-Hsiung Lin, Bin-Nan Wu, Kun-Tai Lee, Wen-Ter Laisections were incubated with 3% H2 O2 for 10 min at room temperature, blocked with blocking buffer (ab126587, Abcam, Cambridge, MA, USA) and incubated overnight at 4 ◦ C with primary antibodies targeting Cx40

(12) Simulated Microgravity Inhibits the Proliferation of Chang Liver Cells by Attenuation of the Major Cell Cycle Regulators and Cytoskeletal ProteinsInternational Journal of Molecular SciencesApril 27, 2021Chi Nguyen Quynh Ho, Minh Thi Tran, Chung Chinh Doan, Son Nghia Hoang, Diem Hong Tran, Long Thanh Le(ab133411, Abcam, Cambridge, MA, USA), and the membrane was blocked overnight at 4 ◦ C with blocking buffer (ab126587, Abcam, Cambridge, MA, USA). The membrane was incubated with primary antibodies in blocking buffer overnight

(13) Lipid bubbles combined with low-intensity ultrasound enhance the intratumoral accumulation and antitumor effect of pegylated liposomal doxorubicin in vivoDrug DeliveryMarch 9, 2021Inoru Yokoe, Daiki Omata, Johan Unga, Ryo Suzuki, Kazuo Maruyama, Yoshiharu Okamoto, Tomohiro Osakiwere prepared using a cryostat and blocked using 0.1% Tween-20 in a commercial blocking buffer (abcam ab126587, Abcam, Tokyo, Japan)-PBS solution for 1 h at RT. The sections were incubated with rat anti-mouse CD31

(14) Gradient Waveguide Thickness Guided-Mode Resonance BiosensorSensors (Basel)January 7, 2021Jia-Ming Yang, Nien-Zu Yang, Cheng-Hao Chen, Cheng-Sheng Huangto remove unbounded antibodies. The measurement channel was then blocked in a solution of 1% casein (ab126587, Abcam) in PBS for 1 h at room temperature to minimize nonspecific binding during antigen incubation.

(15) YQWY Decoction Improves Myocardial Remodeling via Activating the IL-10/Stat3 Signaling PathwayEvidence-Based Complementary and Alternative MedicineDecember 14, 2020He Li, Zhi-Jun Gong, Yun He, Jing-Jing Huang, Yu-Ning Jiang, Ya-Yang Liu, Yao Zhu, Wei-Min JiangAfter antigen retrieval in citrate, the sections were incubated with protein blocking buﬀer (pH 7.5) (ab126587, Abcam) for an hour at room temperature (RT). The samples were then incubated with speciﬁc primary antibodies

(16) Simulated Microgravity Reduces Proliferation and Reorganizes the Cytoskeleton of Human Umbilical Cord Mesenchymal Stem CellsPhysiological ResearchOctober 31, 2020H N Quynh Chi, H Nghia Son, D Chinh Chung, L D HUAN, T Hong Diem, L T LONGtransferred to a PVDF membrane (ab133411; Abcam), which was blocked overnight at 4 °C with a blocking buffer (ab126587; Abcam). The membrane was then incubated with primary antibodies in the blocking buffer overnight at

(17) Discoidin Domain Receptor 1 Regulates Runx2 during Osteogenesis of Osteoblasts and Promotes Bone Ossification via Phosphorylation of p38International journal of molecular sciencesSeptember 29, 2020Chou LY, Chen CH, Chuang SC, Cheng TL, Lin YH, Chou HC, Fu YC, Wang YH, Wang CZ.were incubated with 3% H2O2 peroxidase for 10 min at room temperature, blocked with blocking buffer (ab126587, Abcam, Cambridge, MA, USA) and incubated overnight at 4 °C with primary antibodies targeting DDR1 (PA5-29316,

(18) Low-Intensity Pulsed Ultrasound Alleviates Hypoxia-Induced Chondrocyte Damage in Temporomandibular Disorders by Modulating the Hypoxia-Inducible Factor PathwayFrontiers in PharmacologyMay 14, 2020Tao Yang, Chao Liang, Lei Chen, Jun Li, Wei Gengfor 20 min, permeabilized with 0.2% triton X-100 for 10 min, and then incubated with blocking buffer (ab126587, Abcam) for 1 h. Samples were incubated with primary antibodies (i.e. anti-Col 2; 1:200, ab34712, Abcam)

(19) The Borrelia burgdorferi VlsE Lipoprotein Prevents Antibody Binding to an Arthritis-Related Surface AntigenCell ReportsMarch 17, 2020Abdul G. Lone, Troy BankheadA9528: CAS: 1397-89-3 Betadine Surgical Scrub,Purdue Products,Cat# 516163 Blocking buffer,Abcam,Cat# ab126587 Clarity Western ECL,Bio-Rad Laboratories,Cat# 170-5060 Gentamycin,Sigma,Cat# G1264; CAS: 1405-41-0 Isopropyl

(20) MicroRNA‑107 may regulate lung cancer cell proliferation and apoptosis by targeting TP53 regulated inhibitor of apoptosis 1Oncology LettersJanuary 7, 2020Peng Cai, Jingjing Li, Guiming Chen, Bing Peng, Liuyang Yu, Bolin Zhao, Yi Yucat. no. ab9485; Abcam). All antibodies were diluted in blocking buffer (concentration, 10x; cat. no. ab126587; Abcam). After washing, the membrane was then incubated for 1 h at 37˚C with goat anti‑rabbit horseradish peroxidase

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10X Blocking Buffer from Abcam

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